L-malic acid is oxidised by nicotinamide adenine dinucleotide (NAD) to oxaloacetate using L-malate dehydrogenase (MDH) enzyme as a catalyst. The equilibrium does not favour formation of
oxaloacetate and so oxaloacetate is removed by a trapping enzyme. The amount of NADH formed is measured at 340 nm and is stoichiometrically related to the amount of L-malate consumed. In this method, glutamate oxaloacetate transaminase (GOT) is used as the trapping enzyme. In the presence of L-glutamate, the oxaloacetate is irreversibly converted to L-aspartate.
oxaloacetate and so oxaloacetate is removed by a trapping enzyme. The amount of NADH formed is measured at 340 nm and is stoichiometrically related to the amount of L-malate consumed. In this method, glutamate oxaloacetate transaminase (GOT) is used as the trapping enzyme. In the presence of L-glutamate, the oxaloacetate is irreversibly converted to L-aspartate.
L-Malic Acid 30 Test kit
Product Code: 4A160
Please note these tests require the use of a spectrophotometer.
| Reagent No. | Reagent | Preparation | Quantity | Stability |
| 1 | Buffer | Nil | 33 mL | 2 years at 4°C |
| 2 | NAD | Add 6.6 mL of distilled water and mix by inversion to dissolve | 6.6 mL | 2 years at 4°C as powder (once diluted: 1 year at 4°C) |
| 3 | GOT | Swirl gently before use | 0.4 mL | 2 years at 4°C |
| 4 | MDH | Swirl gently before use | 0.4 mL | 2 years at 4°C |
| 5 | Standard | Nil | 3.3 mL | 2 years at 4°C |
L-Malic Acid 100 Test kit
Product Code: 4A165
Please note these tests require the use of a spectrophotometer.
| Reagent No. | Reagent | Preparation | Quantity | Stability |
| 1 | Buffer | Nil | 2 x 53 mL | 2 years at 4°C |
| 2 | NAD | Add 22 mL of distilled water and mix by inversion to dissolve | 22 mL | 2 years at 4°C as powder (once diluted: 1 year at 4°C) |
| 3 | GOT | Swirl gently before use | 1.3 mL | 2 years at 4°C |
| 4 | MDH | Swirl gently before use | 1.3 mL | 2 years at 4°C |
| 5 | Standard | Nil | 3.3 mL | 2 years at 4°C |
L-Malic Acid Auto Test Kit
Product Code: 4B160
This kit has been designed to work perfectly with Vintessential’s new Chemwell Discrete Analysers (and Thermo’s Arena and Gallery Analysers). All the volumes are set to run out at the same time, so you won’t suffer any wastage.
Malo-lactic fermentation occurs in wine when MLF bacteria convert malic acid to lactic acid. This spectrophotometric assay accurately determines the levels of malic acid, allowing MLF progress to be monitored and ensuring that conversion is complete.
| Reagent No. | Reagent | Quantity | Stability |
| REAGENT 1 | Buffer | 19.5mL x 2 | 24 months at 4°C |
| REAGENT 2 | NAD | 5.5mL x 2 | 12 months at 4°C |
| REAGENT 3 | GOT/MDH | 5.5mL x 2 | 9 months at 4°C |
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