Vintessential: Malic Acid Test Kits for Wine

Malo-lactic fermentation occurs in wine when MLF bacteria convert malic acid to lactic acid. This spectrophotometric assay accurately determines the levels of malic acid, allowing MLF progress to be monitored and ensuring that conversion is complete.

L-malic acid is oxidised by nicotinamide adenine dinucleotide (NAD) to oxaloacetate using L-malate dehydrogenase (MDH) enzyme as a catalyst. The equilibrium does not favour formation of
oxaloacetate and so oxaloacetate is removed by a trapping enzyme. The amount of NADH formed is measured at 340 nm and is stoichiometrically related to the amount of L-malate consumed. In this method, glutamate oxaloacetate transaminase (GOT) is used as the trapping enzyme. In the presence of L-glutamate, the oxaloacetate is irreversibly converted to L-aspartate.

L-Malic Acid 30 Test kit

Product Code: 4A160

Please note these tests require the use of a spectrophotometer.

Reagent No.ReagentPreparationQuantityStability
1BufferNil33 mL2 years at 4°C
2NADAdd 6.6 mL of distilled water and mix by inversion to dissolve6.6 mL2 years at 4°C as powder (once diluted: 1 year at 4°C)
3GOTSwirl gently before use0.4 mL2 years at 4°C
4MDHSwirl gently before use0.4 mL2 years at 4°C
5StandardNil3.3 mL2 years at 4°C

L-Malic Acid 100 Test kit

Product Code: 4A165

Please note these tests require the use of a spectrophotometer.

Reagent No.ReagentPreparationQuantityStability
1BufferNil2 x 53 mL2 years at 4°C
2NADAdd 22 mL of distilled water and mix by inversion to dissolve22 mL2 years at 4°C as powder (once diluted: 1 year at 4°C)
3GOTSwirl gently before use1.3 mL2 years at 4°C
4MDHSwirl gently before use1.3 mL2 years at 4°C
5StandardNil3.3 mL2 years at 4°C

L-Malic Acid Auto Test Kit

Product Code: 4B160

This kit has been designed to work perfectly with Vintessential’s new Chemwell Discrete Analysers (and Thermo’s Arena and Gallery Analysers). All the volumes are set to run out at the same time, so you won’t suffer any wastage.
Malo-lactic fermentation occurs in wine when MLF bacteria convert malic acid to lactic acid. This spectrophotometric assay accurately determines the levels of malic acid, allowing MLF progress to be monitored and ensuring that conversion is complete.
Reagent No.ReagentQuantityStability
REAGENT 1Buffer19.5mL x 224 months at 4°C
REAGENT 2NAD5.5mL x 212 months at 4°C
REAGENT 3GOT/MDH5.5mL x 29 months at 4°C

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