Sigma-Aldrich Classical Cell Culture Media and Buffers For Sale

Classical cell culture media and balanced salt solutions for mammalian cell culture, hybridoma culture, primary cells, and routine laboratory workflows.

Imbros supplies a wide range of Sigma-Aldrich cell culture media and buffers, including DMEM, RPMI 1640, DMEM/F12, MEM, Ham’s nutrient mixtures, Medium 199, DPBS, HBSS and other classical formulations. Available options include liquid and powder formats, with or without L-glutamine, sodium pyruvate, HEPES, glucose, sodium bicarbonate and phenol red.

Choosing the Right Media or Buffer

Cell type: adherent, suspension, primary, hybridoma or specialised lines
Glucose level: high glucose, low glucose or glucose-free formulations
L-glutamine format: with L-glutamine, without L-glutamine or stable glutamine
Phenol red: useful pH indicator, but not always suitable for hormone-sensitive assays
Buffer system: bicarbonate, HEPES or application-specific formulations
Format: ready-to-use sterile liquid or economical powdered media

DMEM - Dulbecco’s Modified Eagle Medium

DMEM is one of the most widely used media for adherent mammalian cell culture. Sigma-Aldrich offers high glucose, low glucose, glucose-free, L-glutamine, sodium pyruvate, HEPES, phenol red-free, liquid and powder formulations.

Product Code	Format / Formulation	Description
D6429	DMEM, high glucose	With sodium bicarbonate, L-glutamine and sodium pyruvate; liquid, sterile-filtered, suitable for cell culture.
D5796	DMEM, high glucose	With sodium bicarbonate and L-glutamine, without sodium pyruvate; liquid, sterile-filtered.
D6046	DMEM, low glucose	With sodium bicarbonate, sodium pyruvate and L-glutamine; liquid, sterile-filtered.
D6546	DMEM, high glucose	With sodium bicarbonate and sodium pyruvate, without L-glutamine; liquid, sterile-filtered.
D5671	DMEM, high glucose	With sodium bicarbonate, without L-glutamine and sodium pyruvate; liquid, sterile-filtered.

RPMI 1640 Media

RPMI 1640 is commonly used for non-adherent cells, including lymphocytes, blood-derived cells, hybridomas and other suspension culture workflows.

Product Code	Format / Formulation	Description
R8758	RPMI 1640	Commonly used RPMI formulation for mammalian cell culture workflows.
R0883	RPMI 1640	Alternative RPMI formulation; suitable for suspension and blood-derived cell culture.
R7388	RPMI 1640	RPMI formulation option for routine cell culture applications.

DMEM/F12 Media

DMEM/F12 is a 1:1 mixture of DMEM and Ham’s F-12, commonly used in serum-free, primary cell and specialised mammalian cell culture applications. HEPES-buffered options are available for improved buffering stability.

Product Code	Format / Formulation	Description
D8437	DMEM/F12	Classical DMEM/F12 formulation for mammalian cell culture.
D6421	DMEM/F12	Alternative DMEM/F12 formulation for specialised culture workflows.
D8900	DMEM/F12	DMEM/F12 format suitable for routine and specialised mammalian culture.

MEM - Minimum Essential Medium

MEM is a widely used synthetic medium for monolayer cultures and general mammalian cell culture. Formulations may include Earle’s or Hanks’ salts, non-essential amino acids, L-glutamine or calcium-free options depending on application.

Product Code	Format / Formulation	Description
M4655	MEM	Minimum Essential Medium formulation for routine mammalian cell culture.
M2279	MEM	Alternative MEM format for monolayer cell culture applications.

Balanced Salt Solutions - DPBS, HBSS & EBSS

Balanced salt solutions are used for washing, dilution, transport and maintaining osmotic balance during cell culture workflows. Common options include DPBS, Hanks’ balanced salt solution and Earle’s balanced salt solution.

Product Code	Buffer / Salt Solution	Description
D8537	DPBS	Dulbecco’s phosphate buffered saline for washing and cell culture handling.
H6648	HBSS	Hanks’ balanced salt solution for cell culture and sample handling workflows.
E2888	EBSS	Earle’s balanced salt solution for routine cell culture applications.

Other Classical Media

Additional classical media options include Ham’s F-10, Ham’s F-12, Medium 199, IMDM, L-15, McCoy’s 5A and other specialised formulations for primary cells, hybridomas, hepatocytes, vaccine production and research applications.

Product Code	Media Type	Description
I3390	IMDM	With sodium bicarbonate, without L-glutamine; liquid, sterile-filtered.
I6529	IMDM	With sodium bicarbonate and L-glutamine; liquid, sterile-filtered.
M199	Medium 199	Classical medium commonly used in virology, vaccine production and primary tissue culture.

Need help selecting the right formulation?
Imbros can assist with selecting media based on cell type, glucose level, glutamine requirement, phenol red preference, buffering system and format.