High-performance ultrafiltration devices for protein concentration and sample preparation
Amicon® Ultra centrifugal filter devices are trusted worldwide for rapid protein concentration, desalting, and buffer exchange applications. Designed with high-recovery Ultracel® regenerated cellulose membranes and a vertical membrane orientation, Amicon® filters deliver fast processing times, high concentration factors, and excellent sample recovery even from dilute or complex solutions.
Available in multiple sample volumes and molecular weight cut-offs (MWCO), Amicon® Ultra filters are suitable for protein purification, nucleic acid preparation, antibody concentration, biomolecule recovery, and cell lysate cleanup workflows. Their low-binding membrane chemistry helps minimise sample loss while maintaining reproducible filtration performance.
- High sample recovery: typically greater than 90%
- Fast processing times: vertical membrane design reduces concentration polarization
- Low nonspecific binding: Ultracel® regenerated cellulose membrane chemistry
- Broad MWCO range: available in 3 kDa, 10 kDa, 30 kDa, 50 kDa and 100 kDa
- Multiple sample volumes: 0.5 mL, 2 mL, 4 mL and 15 mL formats available
- Easy concentrate recovery: reverse spin capability on selected formats
- Excellent reproducibility: reliable ultrafiltration performance across workflows
- Compatible with protein and nucleic acid applications
Researchers commonly choose Amicon® Ultra filters for protein purification, concentration of antibodies and enzymes, sample cleanup prior to chromatography or electrophoresis, and nucleic acid preparation. Their consistent membrane performance and reliable concentration factors make them a standard choice in molecular biology, proteomics, and biotechnology laboratories.
One of the major concerns with centrifugal ultrafiltration devices is sample loss caused by membrane binding or excessive concentration polarization. Amicon® Ultra filters address this through their vertical membrane design and low-binding Ultracel® membrane technology, helping maximise sample recovery while reducing spin times.
Technical Specifications
| Membrane Material | Ultracel® regenerated cellulose |
| MWCO Options | 3 kDa, 10 kDa, 30 kDa, 50 kDa, 100 kDa |
| Available Volumes | 0.5 mL, 2 mL, 4 mL, 15 mL |
| Applications | Protein concentration, desalting, buffer exchange, nucleic acid preparation |
| Sample Recovery | Typically >90% |
| Sterility | Non-sterile |
| Binding Characteristics | Low nonspecific binding |
| Processing Time | Typically 10–30 minutes depending on sample and volume |
Available Amicon® Ultra Centrifugal Filters
| Product Code | Sample Volume | MWCO | Description |
|---|---|---|---|
| UFC500324 | 0.5 mL | 3 kDa | Amicon® Ultra-0.5 centrifugal filter with Ultracel® regenerated cellulose membrane. |
| UFC501024 | 0.5 mL | 10 kDa | Low-binding ultrafiltration device for protein concentration and desalting. |
| UFC503024 | 0.5 mL | 30 kDa | Fast-processing centrifugal filter for biomolecule concentration workflows. |
| UFC505024 | 0.5 mL | 50 kDa | Ultrafiltration device designed for rapid sample concentration. |
| UFC510024 | 0.5 mL | 100 kDa | High molecular weight cutoff centrifugal ultrafiltration filter. |
| UFC200324 | 2 mL | 3 kDa | 2 mL centrifugal filter device for concentration and buffer exchange. |
| UFC203024 | 2 mL | 30 kDa | Ultrafiltration device suitable for protein purification workflows. |
| UFC205024 | 2 mL | 50 kDa | Low-binding regenerated cellulose membrane centrifugal filter. |
| UFC210024 | 2 mL | 100 kDa | High MWCO centrifugal filter for large biomolecule concentration. |
| UFC800324 | 4 mL | 3 kDa | 4 mL Amicon® Ultra filter for rapid ultrafiltration workflows. |
| UFC801024 | 4 mL | 10 kDa | Protein concentration filter with high sample recovery. |
| UFC803024 | 4 mL | 30 kDa | Ultrafiltration device for concentration and desalting applications. |
| UFC805024 | 4 mL | 50 kDa | Fast-processing regenerated cellulose centrifugal filter. |
| UFC810024 | 4 mL | 100 kDa | 4 mL centrifugal ultrafiltration filter with high MWCO. |
| UFC900324 | 15 mL | 3 kDa | Large-volume centrifugal filter device for protein concentration. |
| UFC901024 | 15 mL | 10 kDa | 15 mL ultrafiltration device suitable for biomolecule purification. |
| UFC903024 | 15 mL | 30 kDa | High-capacity centrifugal filter for desalting and concentration. |
| UFC905024 | 15 mL | 50 kDa | Low-binding Amicon® Ultra filter for protein workflows. |
| UFC910024 | 15 mL | 100 kDa | Large-volume ultrafiltration device with high molecular weight cutoff. |
