Dartmouth Ocean: eDNA Sampler

Autonomous eDNA Multi-sampling for the Detection of Biological Genomic Signatures in Water

Environmental DNA Sampling

Autonomous eDNA Multi-sampling for the Detection of Biological Genomic Signatures in Water

  • – Automatic operation according to a preconfigured mission profile
  • – Micro-fluidic architecture reduces reagent usage and facilitates storage of waste products for later disposal
  • – Sample preservation via RNAlater enables long-term, unsupervised deployment
  • – Customize based on the filter material needed (ie. PC, PVDF)
  • – Optional: Integrated 4-channel fluorometer (Chl-a, PC, PE, FDOM)
  • – Compact design allows for deployment from a variety of vessels and platforms

Multi-sample eDNA Collection Made Easy

eDNA sampling is a valuable tool for studying both prokaryotic and eukaryotic biodiversity in the water column.​  Dartmouth Ocean Technologies has developed a portable, convenient solution for use in many data collection scenarios.

The programmable nature of the instrument allows for increased sampling resolution over long periods of time – ideal for time series studies​.

DNA can be extracted from filters using various commercial kits (ie. Qiagen, BioMeme)​. The integrated multi-sample holder makes transporting the samples more convenient.

Environmental Monitoring

Commissioning and decommissioning of various coastal and offshore work sites​:

  • – Oil and Gas
  • – Wind farms
  • – Aquaculture

Monitoring the environment for specific organisms of interest:

  • – Harmful algae (ie. Alexandrium sp.)
  • – Parasites or pathogens
  • – Indicator species

DNA extracted from the sampler filters can be further processed with methods such as qPCR​

Specifications

Weight: 

Standard:
In Air: 10.1 kg (22.2 lbs)
In Salt Water: 0.53 kg (1.2 lbs)

Extra Capacity:
In Air: 10.2 kg (22.4 lbs)
In Salt Water: 0.53 kg (1.2 lbs)

Deep Water:
In Air: 10.3 kg (22.2 lbs)
In Salt Water: 0.54 kg (1.2 lbs)

Dimensions: 

Diameter: 167 mm (6.6 in)
Length:
Standard: 721 mm (28.4 in)
Extra Capacity: 800 mm (31.5 in)
Deep Water: 800 mm (31.5 in)
+ compensator

Sample Filters (defaults):

– Size: 25mm
– Pore Size: 0.2μm to 2.0μm
– Material: Polycarbonate (PC)

Sample Rate: 20 to 90 minutes per filter (volume dependent)

Sample Volume: Tested for 100 – 500 ml (user-definable)

Intake Filter: 35μm PEEK (default)

Data Output: RS232

Reagents: (volumes per filter) 5% HCl (14 ml) RNAlater (6 ml) Milli-Q Water (42 ml)

Depth Rating: 20 metres

Power Draw: 7VDC or 24VDC

Filters per Cartridge: 9

Download Brochure More information from Dartmouth Ocean Technologies

Make An Enquiry