Environmental DNA Sampling
Autonomous eDNA Multi-sampling for the Detection of Biological Genomic Signatures in Water
- – Automatic operation according to a preconfigured mission profile
- – Micro-fluidic architecture reduces reagent usage and facilitates storage of waste products for later disposal
- – Sample preservation via RNAlater enables long-term, unsupervised deployment
- – Customize based on the filter material needed (ie. PC, PVDF)
- – Optional: Integrated 4-channel fluorometer (Chl-a, PC, PE, FDOM)
- – Compact design allows for deployment from a variety of vessels and platforms
Multi-sample eDNA Collection Made Easy
eDNA sampling is a valuable tool for studying both prokaryotic and eukaryotic biodiversity in the water column. Dartmouth Ocean Technologies has developed a portable, convenient solution for use in many data collection scenarios.
The programmable nature of the instrument allows for increased sampling resolution over long periods of time – ideal for time series studies.
DNA can be extracted from filters using various commercial kits (ie. Qiagen, BioMeme). The integrated multi-sample holder makes transporting the samples more convenient.
Environmental Monitoring
Commissioning and decommissioning of various coastal and offshore work sites:
- – Oil and Gas
- – Wind farms
- – Aquaculture
Monitoring the environment for specific organisms of interest:
- – Harmful algae (ie. Alexandrium sp.)
- – Parasites or pathogens
- – Indicator species
DNA extracted from the sampler filters can be further processed with methods such as qPCR
Specifications
Weight:
Standard:
In Air: 10.1 kg (22.2 lbs)
In Salt Water: 0.53 kg (1.2 lbs)
Extra Capacity:
In Air: 10.2 kg (22.4 lbs)
In Salt Water: 0.53 kg (1.2 lbs)
Deep Water:
In Air: 10.3 kg (22.2 lbs)
In Salt Water: 0.54 kg (1.2 lbs)
Dimensions:
Diameter: 167 mm (6.6 in)
Length:
Standard: 721 mm (28.4 in)
Extra Capacity: 800 mm (31.5 in)
Deep Water: 800 mm (31.5 in)
+ compensator
Sample Filters (defaults):
– Size: 25mm
– Pore Size: 0.2μm to 2.0μm
– Material: Polycarbonate (PC)
Sample Rate: 20 to 90 minutes per filter (volume dependent)
Sample Volume: Tested for 100 – 500 ml (user-definable)
Intake Filter: 35μm PEEK (default)
Data Output: RS232
Reagents: (volumes per filter) 5% HCl (14 ml) RNAlater (6 ml) Milli-Q Water (42 ml)
Depth Rating: 20 metres
Power Draw: 7VDC or 24VDC
Filters per Cartridge: 9