Countess 3 Automated Cell Counter

The Invitrogen Countess 3 Automated Cell Counter uses advanced machine-learning image analysis algorithms to deliver accurate cell counts and viability in less than 30 seconds. The Countess 3 Automated Cell Counter has automated lighting, focus, capturing, counting, and saving. All that needs to be done is to insert the slide with a cell sample.

Thermo Fisher Scientific offers two high-performance cell counters designed to meet the needs of any lab. The Invitrogen™ Countess™ 3 and Countess™ 3 FL Automated Cell Counters utilize artificial intelligence, applying a deep-learning neural network algorithm to generate highly accurate cell counts even for challenging cell sample types. The machine-learning algorithm was taught to the systems by our scientists to help ensure accuracy and precise cell counting, and avoid the user variation associated with manual hemocytometer cell counting.

The Countess 3 Automated Cell Counters come equipped with automated features like autolighting, autofocus, and autosave to provide convenience and time savings.


Highly accurate machine-learning algorithm applied to cell counting


Auto-lighting and autofocus minimize user inputs and variability

Time savings

Rapid capture and autosave features help generate and save results fast


Built-in pre-dilution and cell splitting calculators, histograms and gating, and a PDF report of results


Both Countess 3 instrument models are compatible with the Invitrogen™ Countess™ Reusable Slide

Start counting cells in 3 simple steps

Step 1

Mix equal parts sample with 0.4% Trypan Blue solution and pipet 10µL into a Countess chamber slide or reusable slide.

Step 2

Enable ‘Rapid Capture’ from the Home Screen.

Step 3

Insert slide into the instrument until it clicks into position.

Countess 3 FL Automated Cell Counter

Fluorescence detection beyond GFP and RFP
The Countess 3 FL Automated Cell Counter offers one brightfield and two interchangeable fluorescence channels. The fluorescence colors detected are determined by the insertion of individual light cubes. The wide variety of light cubes available provides flexibility for multiple research applications from UV-excitable DAPI to far-red.

Interchangeable LED light cubes
At the heart of the Countess system’s fluorescence technology are the unique Invitrogen™ EVOS™ Light Cubes. Each cube contains an LED, illuminating optics, and filters. Light cubes are user-interchangeable and automatically detected by the Countess 3 FL system for plug-and-play ease. There are ample light cubes to choose from.

Servicing, Calibration and Support

Imbros’ local engineering department can service, repair and calibrate your instrument without expensive shipping costs or wait times.


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